Transcriptome data of Thitarodes xiaojinensis larvae infected with entomopathogenic fungi

Sixth-instar Thitarodes xiaojinensis larvae were infected with the blastospores of Ophiocordyceps sinensis (Os), Cordyceps militarist (Cm), or Beauveria bassiana (Bb). Larvae injected with Ringer’s buffer (RS, 8.05 g NaCl, 0.42 g KCl, and 0.18 g CaCl2 per liter) were used as the control. Each larva was injected with 2 µL of Ringer’s buffer or blastospore suspension in Ringer’s buffer (1.0 × 107 cells/mL) using glass capillaries. Each treatment included 12 larvae. According to the manufacturer’s instructions, total RNA from the fat body of control and fungus-infected larvae was extracted at 72 hpi using TRIzol reagent (Invitrogen, USA). RNA yields were quantified using a NanoDrop 2000 spectrophotometer. RNA integrity was confirmed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system. A cDNA library was constructed using the total RNA and sequenced with 150 bp paired-end reads on an Illumina NovaSeq platform. Three biological replicates were used for each treatment.

本实验中，第六龄 Thitarodes xiaojinensis 蠕虫感染了虫草真菌 Ophiocordyceps sinensis (Os)、Cordyceps militarist (Cm) 或 Beauveria bassiana (Bb) 的子囊孢子。以林格氏溶液（RS，每升含 8.05 克氯化钠、0.42 克氯化钾和 0.18 克氯化钙）注射的蠕虫作为对照组。每只蠕虫通过玻璃毛细管注入 2 微升林格氏溶液或林格氏溶液中的子囊孢子悬浮液（1.0 × 10^7 细胞/毫升）。每个处理组包含 12 只蠕虫。根据制造商的说明，在感染后 72 小时，使用 TRIzol 试剂（Invitrogen，美国）从对照组和真菌感染组蠕虫的脂肪体中提取总 RNA。RNA 产量使用 NanoDrop 2000 分光光度计进行量化。使用 Bioanalyzer 2100 系统的 RNA Nano 6000 测定试剂盒确认 RNA 完整性。利用总 RNA 构建cDNA文库，并在Illumina NovaSeq平台上进行 150 碱基配对末端读取测序。每个处理组使用三个生物重复。