Additional file 4: Figure S5. of Mice lacking the conserved transcription factor Grainyhead-like 3 (Grhl3) display increased apposition of the frontal and parietal bones during embryonic development

Analysis of gene expression in the developing coronal sutures of WT and Grhl3−/− embryos at E18.5. (A-B) The expression of β-galactosidase (corresponding to the LacZ transgene inserted within the Grhl3 locus, simultaneously disrupting gene function, as well as acting as a reporter to confirm presence of the deleted allele [7]) was used to confirm loss of Grhl3. The expression of total FGFR1 (C-D), pFGFR1 (E-F), FGFR2 (G-H), Twist (I-J), Runx2 (K-L) and Noggin (M-N) was examined by immunohistochemical analysis. Other than an apparent loss of pFGFR1 in the most superficial layers of the surface ectoderm in Grhl3 −/− embryos at E18.5 (compare E with F; arrowheads), no significant differences in the expression of any of these genes was detected at E18.5, within the calvaria, dura or suture regions. (ZIP 362 kb)