Signaling induced biophysical disruption of repressed chromatin domains drives immune cell fate

CUT&Tag samples using anti-H3K36me2 in total germinal center cells, resting (0h), stimulated (18h). Stimulation conditions were as follows: 10 µg/ml anti-CD40 (Thermo Fisher Scientific 16-0402-86), 10 µg/ml anti-IgG (Thermo Fisher Scientific 16-5098-85), 10 µg/ml anti-IgM (Jackson ImmunoResearch 115-005-020) and 5 ng/ml IL-21 (R&D Systems 594-ML-010). CUT&Tag samples using anti H3K27me3, H3K27ac, H3K4me1, H3K4me3 and H3.3 in resting and stimulated naive B cells (conditions as for GCB but omitting anti-IgG). CUT&Tag samples using anti-H3K27me3 and ChIP using anti H3S28ph in NK cells (resting and stimulated for 1 and 24 hours with Il-12, Il-18 and il-15). CUT&Tag samples using anti-H3K27me3 and ChIP using anti H3S28ph in naive CD4 T cells (resting and stimulated for 4 and 24 hours with anti CD3, anti CD28 and IL-2).