Long non-coding RNA and mRNA expression profiles in rats with LPS-induced myocardial dysfunction. Long non-coding RNA and mRNA expression profiles in rats with LPS-induced myocardial dysfunction

Sepsis is a life-threatening disease that often causes multiple organ dysfunctions. Long noncoding RNAs (lncRNAs) are involved in the pathogenesis and development of sepsis. However, there is little known about the function of lncRNAs in sepsis-induced myocardial dysfunction.To test the hypothesis that dysregulating lncRNAs may be involved in sepsis-induced myocardial dysfunction, we identified differentially expressed (DE) lncRNAs and mRNAs in cardiac tissue by high-throughput sequencing. We constructed coding and non-coding co-expression (CNC) and lncRNA-micro RNA (miRNA)-mRNA competing endogenous networks. The findings will provide useful information for exploring therapeutic candidate targets and new molecular biomarkers of septic myocardial injury. Overall design: Male Wistar rats (8–10 weeks old, 150–200 g) were randomly divided into the following two groups: rats in the LPS group (n = 5) received an intraperitoneal injection of 10mg/kg LPS (5 mg LPS dissolved in 1 mL 0.9% saline), and those in the control group (n = 5) were intraperitoneally injected with 0.9% saline (2 ml/kg). we analyzed the lncRNAs, miRNAs and mRNAs expression profiles using RNA-seq. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were carried out and coding and non-coding co-expression and competing endogenous RNA networks were constructed to investigate the mechanisms.