Distances table showing the pairwise genetic and amino acid distances of an alignment of 26 LIV genomes, an alignment of 36 TBEV-Eu genomes, and an alignment combining both datasets.

Mean distances are shown with the range highlighted in brackets. The alignments were generated, and distance tables generated using MUSCLE within the Geneious software package. As some sequences contained gaps at the 5’ and 3’ genomic termini, only complete genomes were utilised for comparison of the full genome sequence, and the 5’ and 3’ UTRs. For LIV 21 sequences were utilised for the 5’ UTR, 22 for the 3’ UTR and 21 for the full genome sequence. For TBEV-Eu 26 sequences were utilised for the 5’ UTR, 20 for the 3’UTR, 22 for the full genome nucleotide sequence and 36 genomes for the amino acid sequence.