scRNA-seq analysis of liver constituent cells from human FALD and normal livers

scRNA-seq was performed on Fontan-associated liver disease (FALD) and normal livers using 10x Genomics Fixed RNA Profiling. The expression of connective tissue growth factor (CTGF) was upregulated in the EC cluster in the FALD livers. The scRNA-seq analysis also showed that the integrin signaling pathway was activated and the expression of YAP/TAZ target genes was upregulated in the EC cluster in the FALD livers. Overall design: Frozen surgical liver tissue was cut into pieces approximately 1 mm3, fixed with formalin using a Chromium Next GEM Single-Cell Fixed RNA Sample Preparation Kit (10x Genomics), and then dispersed into single cells using gentleMACS (Miltenyi Biotec) according to the manufacturer's instructions.