Environmental metabarcoding for monitoring benthic communities.

The files provided here are the raw fastq files used for the manuscript Laroche et al., 2017 "Metabarcoding monitoring analysis: the pros and cons of using co-extracted environmental DNA and RNA data to assess offshore oil production impacts on benthic communities." which currently under peer-review.Environmental DNA/RNA samples were collected in the Tasman sea (New Zealand) from marine sediment in proximity to an offshore oil production platform. Amplicons were sequenced on an Illumina MiSeq platform.The present files are demultiplexed fastq files of bacteria (16S) and eukaryotes (18S). In file name, BED = DNA sequences and BEC = RNA sequences, the following number (e.g BEC_43...) represent the sample number. R1 = forward sequences, R2 = reverse sequences. The taxa is identified at the end of the name file (e.g. _Bac.fq for bacteria and _Euk.fq for eukaryotes).Please refer to the manuscript for additional information on the dataset.

本处提供之文件为用于Laroche等（2017年）“环境DNA/RNA共提取监测分析：利用环境DNA/RNA数据评估海上石油生产对底栖群落影响的利弊”一文的原始fastq文件。该文目前正在同行评审中。环境DNA/RNA样本采集于新西兰塔斯曼海的海洋沉积物中，位于海上石油生产平台附近。扩增子序列在Illumina MiSeq平台上进行测序。当前文件为细菌（16S）和真核生物（18S）的解复用fastq文件。在文件名中，BED代表DNA序列，BEC代表RNA序列，随后的数字（例如BEC_43...）表示样本编号。R1代表正向序列，R2代表反向序列。物种分类信息位于文件名末尾（例如_Bac.fq表示细菌，_Euk.fq表示真核生物）。请参阅论文以获取有关数据集的更多信息。