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Probing the off-target effect of EGFP siRNA and pro-siRNA in the HeLa-d1EGFP cell line

ChEBI2013-02-12 更新2026-07-21 收录

RNA干扰脱靶效应

细胞基因沉默实验

数据链接：

https://www.ebi.ac.uk/biostudies/ArrayExpress/studies/E-GEOD-44256 数据链接链接失效反馈

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资源简介：

Probing the off-target effect of EGFP siRNA and pro-siRNA in the HeLa-d1EGFP cell line

应用场景：

创建时间：

2013-02-12

RNA干扰脱靶效应

基因沉默优化

Statistical analysis on a randomized genome shows that it is likely that distinct sequences from the same gene map closely elswhere on the genome, demonstrating that non-overlapping dsRNA constructs d

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Reduced seed region-based off-target activity with lentivirus-mediated RNAi

RNA干扰脱靶效应

慢病毒载体

In addition to silencing intended target genes, transfected siRNAs regulate numerous unintended transcripts through a mechanism in which the equivalent of a microRNA-like seed region in the siRNA reco

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shRNA-based miRNA overexpression results in significant off-target effects caused by isomiR production [DU-145 cells]

miRNA功能研究

RNA干扰脱靶效应

miRNA overexpression strategy based on RNA Polymerase (Pol) III mediated expression of short hairpin RNA (shRNA) coding particular miRNA is widely used for miRNA functional studies. For some miRNAs, e

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shRNA off target analysis via RNAseq. Mus musculus

RNA干扰脱靶效应

shRNA功能验证

shRNAs were assessed for off-target effects by comparing the gene expression profiles of cells that they had been infected into. shRNAs designed with the shERWOOD algorithm and house in the ultramir m

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Reduced seed region-based off-target activity with lentivirus-mediated RNAi. Homo sapiens

RNA干扰脱靶效应

慢病毒

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