Summary of rates of cell death examined under increasing levels of cell confluence (0.5 to 20) and under baseline conditions (BC) or transplant conditions (TC).

Flow cytometric analysis of cell suspensions prepared from increasingly confluent cultures examined either immediately following resuspension (baseline conditions, BC) or after undergoing the transplant preparation procedure (transplant conditions, TC). Flasks seeded with 0.5×106 (0.5) and 1×106 (1) cells were pre-confluent at Day 7. Flasks seeded with 5×106 cells (5) were confluent on Day 7. Flasks seeded with 10×106 cells (10) and 20×106 cells (20) resulted in increasingly post-confluent cultures. Cells were stained with annexin V (AnV) and propidium iodide (PI). The data demonstrates that cells from pre-confluent cultures have low levels of total cell death (0.5 BC: 5.6%±1.1%) and that this does not increase following TC (0.5 TC: 4.8%±0.5%). However, cells from post-confluent cultures have progressively increasing levels of cell death (up to 20 TC: 16.5%±0.9%).