Human keratinocytes exposed to tungsten carbide and tungsten carbide cobalt nanoparticles and cobalt chloride

Comparison of transcriptional profiles after exposure of HaCaT cells to WC or WC-Co nanoparticles and respective amount of free cobalt in form of CoCl2 after 3 hours and 3 days of exposure. Genes responding especially to nanoparticles or leached cobalt ions should be determined. Common refernce: pool of 80 RNA Samples of HaCaT cells (treated and not treated), Cy5 labeled. Samples: RNA of HaCaT cells treated 3 hours and 3 days with 30 µg/ml WC nanoparticles, or 33 µg/ml WC-Co nanoparticles (3 µg/ml cobalt content), or 51 µM CoCl2 (equals 3 µg/ml), or water as control, 5 biological replicates for each treatment, all samples were labeled with Cy3 and hybridised together with the common reference.