Relative quantification of 15N labeled acinus-2 pinin-1 and 14N labeled Col through Data Dependent Acquisition to obtain peptide information for targeted Parallel Reaction Monitoring experiment

To examine the properties of 14N and 15N labeled peptides using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer we first set up this DDA experiment. The purpose of this experiment was to choose peptides that are good candidates for targeted quantification. The retention times, mass, and charge from 42 candidate peptides were acquired for targeted quantification.