RNA-seq of NOTCH1 mutation in chronic lymphocytic leukemia cell BaF3.

The hotspot c.7541_7542delCT NOTCH1 mutation has been proven to have a unfavorable clinical impact in chronic lymphocytic leukemia (CLL). The aim of our study was to investigate the influence of NOTCH1 mutation(c.7541_7542delCT) on NOTCH1 signaling in CLL cells using Gene Expression Profiling. Overall design: Examination of mRNA profiles of NOTCH1 genes in BaF3 cells transfected by pmax-Clover/pmax-Clover-ICN/pmax-Clover-ICN mut plasmids.