Transcriptome response study of arabidopsis wild type (Col-0), ERF95 and ERF97 overexpression lines to basal heat stress

Analysis of transcriptome response of heat treated or untreated 7-day old whole seedlings with genotypes: Col-0 (wild type), p35S:ERF95 overexpression line and p35S:ERF97 overexpression line. ERF95 and ERF97 are involved in heat stress response. Results provide insight into the nuclear genes expression profile under heat treatment in Col-0, the nuclear genes expression profile regulated by either ERF95 and ERF97 overexpression before and after heat treatment, and the regulation similarity between ERF95 and ERF97 overexpression lines. Total RNA samples were isolated from 7-day old whole seedlings with genotypes Col-0 (wild type), ERF95 and ERF97 overexpression lines grew under 16 h/8 h light/dark cycle condition with 100 µmol.m-2.s-1 light intensity at 22 °C following with or without 43 °C 22 min heat treatment. mRNA expression profiles were generated by deep sequencing, in duplicate, using Illumina HiSeq 2500.