Mapping nucleosome resolution chromosome folding in yeast by Micro-C

We describe a Hi-C based method, Micro-C, in which micrococcal nuclease is used instead of restriction enzymes to fragment chromatin, enabling nucleosome resolution chromosome folding maps我们介绍了一种基于高通量染色体构象捕获技术（Hi-C）的Micro-C方法，该方法采用微球菌核酸酶（micrococcal nuclease）替代限制性内切酶（restriction enzymes）对染色质进行片段化处理，从而获得核小体分辨率的染色体折叠图谱。