Figure S1 - E2A Predicts Prognosis of Colorectal Cancer Patients and Regulates Cancer Cell Growth by Targeting miR-320a

(A) Left: E2A protein expression of wild type SW480, control SW480, and knocked-down SW480 cells. Right: Change of E2A expression in SW480 cells after transfection of shE2A, E12 or E47: shE2A reduced the expression of E2A in SW480, while E12 and E47 increased E2A expression in SW480/shE2A cells, relative to the controls; (B) Transfection of E12 or E47 inhibited SW480/WT cell growth; (C) E2A regulates cell growth in NCM460 cells; (D) Transfection of E12 or E47 increased G0/G1 phase of SW480/WT cells and decreased the S phase; (E) E2A regulates cell cycle progression in NCM460 cells; (F) Transfection of E12 or E47 upregulated the expression of miR-320a, compared to negative control. Data is expressed as the means ± SD from 3 separate experiments. (*, P<0.05; **, P<0.01).
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