Skeletal Stem Cell Diversity in Mouse Long Bones. Skeletal Stem Cell Diversity in Mouse Long Bones

We have used SmartSeq2 to sequence single phenotypic skeletal stem cell (SSCs) populations purified via FACS from adult mouse long bones. Two putative SSC populations were isolated based on their previously reported surface marker profiles. An osteochondrogenic SSC (ocSSC, CD45-CD31-Tie2-Ter119-CD51+6C3-Thy1-CD105-) and a perivascular SSC (pvSSC, CD45-CD31-Pdgfra+Sca1+CD24+) were investigated. Overall design: SmartSeq2 single cell RNA sequencing of flow cytometrically purified single mouse osteochondral and perivascular skeletal stem cells from adult mouse long bones.