Effect of (R)-bicalutamide, (R)-9 and (S)-11 on BrdU incorporation induced by R1881 in LNCaP cells.

Quiescent LNCaP cells on coverslips were left untreated (control) or treated for 18 hours with the synthetic androgen R1881 (10 nM), in the absence or presence of the indicated antagonists (used at 10 µM or 20 µM). After in vivo pulsing with 100 µM BrdU, BrdU incorporation was analyzed by immunofluorescence and expressed as % of total nuclei. The numbers represent the mean of three independent experiments, with standard deviation t test. No significance was attributed to the difference in BrdU incorporation between the control cells and cells stimulated with 10 nM R1881 in the presence of (R)-bicalutamide (R)-bic or (S)-11 or (R)-9.