RNA-seq of Tetmemena sp.
收藏NIAID Data Ecosystem2026-03-14 收录
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Three bioligcal replicates of total RNA was isolated from asexually growing Tetmemena sp. cells using TRIzol reagent (Thermo Fisher Scientific), and enriched for the poly(A)+ fraction using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Stranded RNA-seq libraries were constructed using the ScriptSeq v2 RNA-seq library preparation kit (Epicentre) and sequenced on an Illumina Nextseq500 at the Columbia Genome Center.
创建时间：
2022-10-05
