Mechanical Measurements of IntI1 Synaptic Complex Stability

These data sets are associated with the publication "The recombination efficiency of the bacterial integron depends on the mechanical stability of the synaptic complex" Preprint available here: https://doi.org/10.1101/2024.04.09.588808

Single-molecule force spectroscopy data of various IntI1 variants forming complexes with different attC-stite variants.

ZIP files contain h5 data recoreded on a LUMICKS C-Trap device.

IntI1wt.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1wt with aadA7bs attC sites

IntI1Y312F-Alanine.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F-double Alanine mutant with aadA7bs attC sites

IntI1Y312F-mEGFP.zip contains correlative force spectroscopy and confocal imaging data (h5 files) of IntI1Y312F-mEGFP binding and moving on single-stranded DNA

IntI1Y312F-Truncated.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F-C-terminal truncation with aadA7bs attC sites

IntI1Y312F_aadA7-bs.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F with aadA7bs attC sites

IntI1Y312F_aadA7-ts.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F with aadA7ts attC sites

IntI1Y312F_aadA7bs-L2.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F with aadA7bs-L2 hybrid attC sites

IntI1Y312F_aadA7bs-VCRwt.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F with aadA7bs-VCRwt hybrid attC sites

IntI1Y312F_L2.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F with L2 attC sites

IntI1Y312F_VCRwt.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F with VCRwt attC sites

IntI1Y312F_VCRinv.zip contains force  spectroscopy data (indivdual h5 traces) of IntI1Y312F with VCRinv attC sites

README.txt contains information about how to read the individual h5 files with e.g. a Python script.