Data_Sheet_1_Metabolomics-based study of the effect of dietary N-carbamoylglutamic acid addition to heifers in late pregnancy on newborn calves.docx

It has been demonstrated that supplementing late-gestation cow diets with NCG (N-carbamoylglutamic acid) increases the serum protein level, boosts immunological function, and increases the birth weight of the calves. However, the underlying mechanism remains unclear. In this experiment, 30 late-gestation Angus heifers almost at same conditions were chosen for this experiment. They were randomly divided into two groups of 15 cows each. A basal diet was provided to the control group, and 30 g/(d-head) of NCG was added to the basal diet of the test group (NCG group). Blood samples were collected from the jugular vein after birth and before the end (when the calves were 90 days old) of the experiment for plasma metabolomics analysis. The metabolomics analysis identified 53 metabolites between the NCG group and control group, with 40 significantly up-regulated and 13 significantly down-regulated. Among them, 33 lipids and lipid-like molecules made up 57.89% of all the metabolites that were found. Thirty-three metabolic pathways enriched by metabolites showed p.adjust

研究表明，在晚孕期奶牛饲料中添加NCG（N-羧基氨基葡萄糖酸）能够提升血清蛋白质水平，增强免疫功能，并增加犊牛的出生体重。然而，其内在机制尚不明确。在本项实验中，选取了30头接近相同生理状态的晚孕期安格斯母牛参与研究。这些母牛被随机分为两组，每组15头。对照组提供基础饲料，而实验组（NCG组）在基础饲料中额外添加了30克/（日·头）的NCG。在出生后和实验结束前（犊牛90日龄时）从颈静脉采集血液样本，用于血浆代谢组学分析。代谢组学分析在NCG组和对照组之间识别出53种代谢物，其中40种显著上调，13种显著下调。在这些代谢物中，由33种脂质及其类似物构成的物质占所有发现的代谢物的57.89%。富含代谢物的33条代谢途径显示了p.adjust值。