RNA-seq Data of Newly-Formed Adipocytes from Adipose Stem Cells of Normal-Weight Polycystic Ovary Syndrome Women vs. Controls

Normal-weight polycystic ovary syndrome (PCOS) women exhibit adipose tissue dysfunction in vivo accompanied by enhanced subcutaneous (SC) abdominal adipose stem cell (ASC) development to adipocytes with greater lipid accumulation per cell in vitro. The goal of this study was to determine whether this phenomenon is associated with abnormal adipogenic gene transcription using RNA-sequencing to examine differential transcription patterns in PCOS vs controls. We found enhanced activation of adipogenic and adipocyte function genes and decreased expression of genes within the Wnt signaling pathway. In this study we conclude that this altered expression of genes in PCOS cells may underlie their exaggerated lipid accumulation in vitro and could predispose to adipose dysfunction in vivo when caloric intake exceeds energy utilization. ASCs were isolated from SC adipose tissue following SC abdominal biopsy in 3 PCOS women and 3 age and BMI matched controls for RNA-sequencing. Second generation of stem cells were cultured until cells reached confluency and expanded for two additional generations until fourth generation. Fourth generation ASCs were then cultured in adipogenic differentiation medium for 3-12 days and isolated for RNA extraction at days 3, 7, and 12. Extracted RNA were processed using Roche KAPA HyperPrep mRNA kit according to manufacturer's instructions and subjected to sequencing on a HiSeq4000 single-end 50 nt.