Reference Short-Read Transcriptomes of Human Peripheral Blood Lymphocytes (Illumina RNA-Seq, Matched to PacBio Iso-Seq Data)

Long-read sequencing technologies such as Iso-Seq (PacBio Inc.) generate highly accurate sequences of full-length mRNA transcript isoforms. Long-read transcriptomics may be especially useful in the context of lymphocyte functional plasticity as it relates to human health and disease. However, no long-read isoform-aware reference transcriptomes of human circulating lymphocytes seem to be publicly available despite being valuable as benchmarks in a variety of transcriptomic studies. To begin to fill this gap, we purified four lymphocyte subsets (CD4 T, CD8 T, NK, and Pan B cells) from the peripheral blood of a healthy male donor and obtained high-quality RNA (RIN>8) for PacBio Iso-Seq analysis and parallel RNA-Seq analysis. Four lymphocyte subsets (CD4 T, CD8 T, NK, and Pan B Cells) were purified from peripheral blood mononuclear cells of a healthy male donor. RNA was extracted from each purified subset and used for Pacific Biosciences Iso-Seq long-read sequencing in parallel with Illumina RNA-Seq short-read sequencing. The resulting datasets are offered here as reference transcriptomes for studies of circulating human lymphocytes.