Single-nuclei RNA-seq of the nucleus tractus solitarii and area postrema at for baseline and LPS-activated cell types

To identify LPS activated cell types in the NTS and AP we region we used single-nuclei RNA seq comparing baseline cell types and cell types labeled using the TRAP2 mouse line crossed to the INTACT mouse line after labeling during LPS treatment. Nuclei were isolated using douce homogenization and stained for NeuN. FACS was used to isolate neuronal nuclei (NeuN+).