Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells: implication in therapy resistance

Gene expression was first compared between prostate adenocarcinoma cell line LNCaP C4-2 and our developed neuroendocrine like derived cell line DKD (LNCaP C4-2 stably depleted with TP53 and RB1). In the next set, we compared the gene expression of neuroendocrine like DKD under presence and absence of Neurolipilin-2. The gene expression was analyzed in triplicate between adeno and neuroendocrine cell lines. Next following Neuropilin-2 depletion by siRNA for 48hrs, gene expression was compared in neuroendocrine cells with control condition.