Sensitive detection of HIV-1 and Mycobacterium tuberculosis peptides in blood for rapid diagnosis of co-infected individuals by immuno-affinity liquid chromatography tandem mass spectrometry

We describe a blood-based immuno-affinity liquid chromatography tandem mass spectrometry (ILM) assay that quantifies proteotypic peptides derived from the Mtb virulence factor CFP10 and the HIV-1 capsid protein p24 to simultaneously diagnose HIV-1 infection and TB disease and to monitor their real-time response to treatment.