RNA-seq of suspension-grown vs. adherent Neisseria gonorrhoeae under conditions of zinc sequestration or zinc excess

Deep sequencing of cDNA of Neisseria gonorrhoeae bacteria that were grown in suspension or adherent to acid-washed glass coverslips, in media where zinc was sequestered or available in excess. Overall design: Total RNA was isolated from Neisseria gonorrhoeae strain FA1090 grown for four hours either in suspension or adherent to acid-washed glass coverslips. Three media conditions were tested: Keratinocyte serum-free medium (KSFM) without additives, KSFM with 3 µM recombinant murine calpotectin, or KSFM with 3 µM mouse calprotectin and 6 µM ZnSO4. Ribosomal RNA was depleted, and remaining RNA was reverse-transcribed into a cDNA library for Illumina sequencing. Reads were mapped to the FA1090 genome.