Transcriptome profiling of five mixed tissues for juvenile Eriocheir sinensis

The transcriptome profiling five tissues of juvenile Eriocheir sinensis, including gill, muscle, thoracic ganglion, eyestalk and hepatopancreas, were sequenced to get the basic dataset for constructed a genome-scale metabolic network model for E. sinensis. The model was used to predict the optimal nutrient requirements of E. sinensis in feed and suggestions for feed improvement were put forward based on the simulation results. Overall design: Five tissues of crabs, including gill, muscle, thoracic ganglion, eyestalk and hepatopancreas, were collected.Total RNA from mixed E. sinensis tissues was sequenced with the Illumina high-throughput sequencing technology by Novogene Inc.The sequenced unigenes were subsequently aligned against the NR, Nt and PFAM databases. GO and KO annotations was obtained by Blast2GO and KAAS (KEGG Automatic Annotation Server).