Effect of IL-7 on CD4+ T cell and Th9 cells. Mus musculus

We found that IL-7 pretreatment enhanced Th9 differentiation. To clarify the underlying mechanisms, we examine the gene expression profiles of CD4+ T cell and Th9 cells with or without IL-7 pretreatment. In Th9 cells, we found that Th9 related genes were greatly increased in IL-7 Th9 group, which demonstrated an enhanced Th9 differentiation. In CD4+ T cells, we found that IL-7 treatment resulted in a global gene expression change especially on chromatin remodeling related genes, which facilitated the entry of transcriptional factor to the Il9 promoter region and promoted Il9 transcription. Overall design: Total RNA obtained from naïve CD4+ T cells or Th9 cells from C57BL/6 mice with or without IL-7 treatment; For CD4+ T cells, IL-7 treated for 0h, 6h or 24h. For Th9 cells, naïve CD4+ T cells were treated for 48h and then cultured for 3 days under Th9 condittion.