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Effect of swab, silica-gel and shock-freezing on the microbiota of the brown alga Saccharina latissima.. Storage Methods

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB37561
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资源简介:
In this study we evaluated the impact of swab, silica-gel or shock-freezing in liquid nitrogen on the Saccharina microbiota. We performed 16S Metabarcoding of the V3-V4 region with the standard Illumina protocol for metabarcoding (Q5® High-Fidelity PCR Kit, AMPure XP for PCR Purification Kit and Nextera XT DNA Library Preparation Kit), and the sequences were obtained with an Illumina MiSeq sequencer using a MiSeq v3 kit (2x300bp, paired-end).
创建时间:
2021-08-27
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