Gene expression in Drosophila melanogaster in response to bacterial infection

The goal of this study was to generate a high-quality dataset of gene expression in Drosophila melanogaster 3 hours after infection with Gram-positive (M luteus) or Gram-negative (E coli) bacteria. Overall design: RNAseq on polyA-selected RNA extracted from whole male adult flies either uninjected or 3 hours after injection with 50nl of either: PBS (vehicle control), E coli suspended in PBS (OD600=1), M luteus suspended in PBS (OD600=1). Flies were kept at 29° after injection on our standard lab diet (10% yeast, 8% fructose, 2% polenta, 0.8% agar, supplemented with nipagin and propionic acid).