Single cell transcriptomic analysis of P8 and P15 mouse cochlea (control and three overexpression conditions)

Purpose - To determine transcriptomic variations in the mouse cochlea in response to three different transcription factor overexpression conditions and no induction control. Methods- SC RNA seq performed on FACS enriched cells obtained from experimental animals (Sox9CreER; Rosa26A/GA/GAP/+; Rosa26 tdtomato). Same samples at two timepoints 1 week (P8) and 2 week (P15) ages. Results- The SC RNA seq highlighted differential transcriptomic changes between the two ages and the three overexpression conditions. Conclusions- Our study highlights the variablitiy in transcriptomic response to three transcription factor combination mediated reprogramming cues at two different ages. The SC RNA seq confirmed a variety of our biological observations with regard to the hair cell reprogramming process in the mouse cochlea. Examination of transcriptome via single cell RNA seq of FACS purified mouse cochlear cells at P8 and P15 induced with transcription factor combinations.