Single cell transcriptomic analysis of P8 and P15 mouse cochlea (control and three overexpression conditions)

Purpose - To determine transcriptomic variations in the mouse cochlea in response to three different transcription factor overexpression conditions and no induction control. Methods- SC RNA seq performed on FACS enriched cells obtained from experimental animals (Sox9CreER; Rosa26A/GA/GAP/+; Rosa26 tdtomato). Same samples at two timepoints 1 week (P8) and 2 week (P15) ages. Results- The SC RNA seq highlighted differential transcriptomic changes between the two ages and the three overexpression conditions. Conclusions- Our study highlights the variablitiy in transcriptomic response to three transcription factor combination mediated reprogramming cues at two different ages. The SC RNA seq confirmed a variety of our biological observations with regard to the hair cell reprogramming process in the mouse cochlea. Examination of transcriptome via single cell RNA seq of FACS purified mouse cochlear cells at P8 and P15 induced with transcription factor combinations.研究目的：探究小鼠耳蜗在三种不同转录因子过表达条件及无诱导对照组中的转录组差异。 实验方法：对来自实验动物（Sox9CreER; Rosa26A/GA/GAP/+; Rosa26 tdTomato）的荧光激活细胞分选（Fluorescence Activated Cell Sorting，FACS）富集细胞进行单细胞RNA测序（Single-Cell RNA Sequencing，SC RNA seq）。实验样本采集于出生后第8天（P8）与出生后第15天（P15）两个时间点。 实验结果：单细胞RNA测序结果表明，两个年龄组与三种过表达条件之间均存在显著的转录组差异变化。 研究结论：本研究揭示了两种不同年龄阶段下，小鼠耳蜗对三种转录因子组合介导的重编程诱导信号的转录组响应差异。单细胞RNA测序验证了我们在小鼠耳蜗毛细胞重编程过程中的多项生物学观察结果。 本研究通过单细胞RNA测序，对经转录因子组合诱导、于P8和P15阶段分离的FACS纯化小鼠耳蜗细胞的转录组进行了分析。