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AP-MS of GFP-MCM2 in the cellular response to etoposide induced DNA damage.

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NIAID Data Ecosystem2026-03-10 收录
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SILAC quantitative proteomics using immunoprecipitation of GFP-tagged fusion proteins to identify proteins interacting with the MCM complex, and quantify changes in interactions in response to DNA damage. U2OS cells expressing GFP-MCM2 were used to purify the MCM complex following treatment or not with etoposide, a topoisomerase II inhibitor causing DNA damage.

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2017-12-06
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