Differences in microbiome populations following hematopoietic stem cell transplantation in Crohn?s disease patients. Differences in microbiome populations following hematopoietic stem cell transplantation in Crohn?s disease patients

Treatment of severely refractory Crohn’s disease (CD) patients remains a clinical challenge. Recent studies show the efficacy of autologous hematopoietic stem cell transplant (HSCT) in these severely compromised patients. HSCT is thought to eliminate auto-reactive cells; however, no specific studies of immune reconstitution in CD patients are available. We studied a group of CD patients (n=18) receiving autologous HSCT. We studied changes in the microbiome induced by HSCT and some non-CD samples. Overall design: Biopsy 16S profiles of CD patients undergoing HSCT were generated by deep sequencing, using MiSeq (Illumina, San Diego, CA)