Time-course of the digestion of prothrombin wildtype and active site mutant S525A by MASP-1.

rMASP-1cf and the different prothrombin forms were incubated for up to 90 min. Bands were identified as a) uncleaved prothrombin, b) prethrombin-1, c) mIIR393 aa1-393, d) prethrombin-2, e) fragment F1.2, f) MASP-1 heavy chain, g) degradation product of the MASP-1 heavy chain, h) C-terminal part of thrombin heavy chain cleaved at R393, i) MASP-1 light chain, j) α-thrombinR393 light chain + heavy chain up to R393, k) fragment F1. Bands not indicated by letters are contaminations of the purification process.