Whole-genome capture and sequencing of Francisella tularensis directly from clinical samples

This dataset comprises: <strong>1. The design of RNA oligonucleotide baits for Agilent Technologies’ SureSelect target enrichment - <strong>54756 RNA oligonucleotide "baits" (120 bp each) </strong></strong>designed to perform <strong>whole-genome capture and sequencing of </strong><strong>Francisella tularensis<strong> directly from clinical samples</strong></strong> (DNA) using Agilent Technologies’ SureSelect target enrichment system following the Illumina paired-end multiplexed sequencing library protocol. RNA oligonucleotide “baits” were designed to span the <em>Francisella tularensis </em>chromosome and plasmid, accounting for the genetic variability among publicly available genome sequences. All resulting sequences were BLASTn searched against the Human Genomic + Transcript database to excluded homologous sequences to the human genome. Overall, a total of 54756 RNA probes were generated and this custom bait library was then uploaded to the SureDesign software (https://earray.chem.agilent.com/suredesign) and synthesized by Agilent Technologies. <strong>2. Genome assemblies of 17 Francisella tularensis samples generated in the context of validation and application of SureSelect target enrichment for Whole-genome capture and sequencing of Francisella tularensis directly from clinical samples </strong> File “<strong>Ft_assembly_metadata.xlsx</strong>” contains the genome assembly statistics for each isolate, including European Nucleotide Archive accession numbers. The archive “<strong>Ft_assemblies.zip</strong>” contains all the genome assemblies (.fasta format) of each isolate presented in the metadata file. More details can be found in the following publication: (available soon)