Additional file 1: of Rhizosheath microbial community assembly of sympatric desert speargrasses is independent of the plant host

Table S1. Soil physico-chemistry of the dune’s bulk sand. All values are given as mean of three replicates ± standard error. Table S2. Measurements of root and rhizosheath diameters (n = 10). Analysis of variance (ANOVA) is reported. For values p < 0.005 post-hoc comparison (Tukey’ test) was done, letters in parenthesis indicate the results of multiple comparisons. Table S3. Results of ANOVA multiple comparison tests analyzing the intraspecific dissimilarity associated to the hosts and bulk sand were reported for (a) bacterial and (b) fungal communities. Average distance from centroid was used as measure of dispersion. Significant differences (p < 0.05) among pair host (speargrasses and bulk sand) were indicated with star (*). Table S4. (a) Estimation of components of variation in bacterial and fungal communities. (b and c) Multi comparison tests (PERMANOVA, number of permutation = 999) for bacterial and fungi, respectively, considering plant species or rhizosheath-root compartments. (d) Mantel test results showing correlations between compositional beta diversity associated to compartments and distance from the dune bottom for both bacteria and fungi. Significance p < 0.05 Table S5. (a) Covariance (ANCOVA) and (b) linear regression analysis of distance decay rates for compositional (Bray-Curtis) similarity in the rhizosheath-root system compartment. Results were reported for bacterial and fungal components. Table S6. Mantel test results showing correlations between phylogenetic alpha-diversity metrics associated to compartments and distance from the dune bottom for both bacteria and fungi. Table S7. Taxonomical classification of (a) bacteria and (b) fungi with relative abundance expressed in percentage. See excel file named Additional file 1: Table S7. Table S8. Evaluation of the effect of single factors ‘Plant species’ and ‘Compartment’ and their interaction (Plant species ´ Compartment) on bacterial and fungal taxonomical distribution using PERMANOVA (main test). Taxonomical distribution have been analyzed at phylum/class and family level for bacteria (99 and 82% sequences classified; a and b, respectively) and at class and genus level for fungi (85 and 70% sequences classified; c and d, respectively). Significant PERMANOVA results (p < 0.05) were indicated with star (*). Table S9. Kruskal-Wallis test to evaluate significant differences in (a) bacterial and (b) fungal relative abundance across groups. See excel file named Additional file 1: Table S9. Table S10. Network table with list of nodes and edge. See excel file named Additional file 1: Table S10. Figure S1. Venn diagram detecting percentage of bacterial and fungal SVs shared among the rhizosheath-root system compartments (root, rhizosheath and rhizosphere) and bulk sand of all the three species studied. Biggest numbers indicate the percentage of SVs and the numbers in parenthesis the relative abundance of those SVs. Figure S2. Venn diagram detecting percentage of bacterial (upper panels) and fungal (lover panels) SVs shared among the rhizosheath-root system compartments (root, rhizosheath and rhizosphere) and bulk sand for each of the three species studied. Biggest numbers indicate the percentage of SVs and the numbers in parenthesis the relative abundance of those SVs. Figure S3. Venn diagram detecting bacterial (upper panels) and fungal (lover panels) SVs shared among the three speargrasses species (S. sabulicola, S. seelyae and C. spinosa) for each rhizosheath-root system compartments (root, rhizosheath and rhizosphere). Biggest numbers indicate the percentage of SV and the numbers in parenthesis the relative abundance of those SVs. Figure S4. Analysis of edge betweenness centrality in speargrasses rhizosheath-root system networks. Color code indicated the interaction among different pair of phylogenetic group. Name of the phylogenetic group are reported in the vertical axis. (ZIP 3591 kb)