The order and stochasticity in the Drosophila genome folding revealed by single-nucleus Hi-C

We generated and analyzed single-cell Hi-C libraries from cultrured Drosophila cells. The data obtained allowed us to explore cell-to-cell variability in TAD profiles and inter-TAD interactions showing that strong TAD boundaries are determined by high levels of active chromatin. DPD polymer simulations were utilized to reconstruct 3D structures of haploid X chromosomes. These structures showed marked cell-to-cell variabilty in the overall shape of the chromosome territory and relatively conserved chromatin chain path inside TADs. DPD polymer simulations were utilized to reconstruct 3D structures of haploid X chromosomes. These structures showed marked cell-to-cell variabilty in the overall shape of the chromosome territory and relatively conserved chromatin chain path inside TADs. Overall design: We generated bulk and single-cell Hi-C libraries for Drosophila melanogaster for 88 ML-DmBG3-c2 cells