Gene expression profiling of CD25High and CD25Low regulatory T cell populations from mice lacking autophagy in their dendritic cells and their littermate controls.

The goal of this study is to compare the transcriptome profiling (RNA-seq) of two CD25 regulatory T cell subsets isolated from secondary lymphoid organs of mice lacking autophagy in their dendritic cells. CD25High and CD25Low regulatory T cells were isolated from Atg5 flox/flox CD11c Cre +/- (Atg5 -/- DC) mice and their littermate controls, Atg5 flox/flox CD11c Cre -/- (Atg5 +/+ DC), under steady state conditions. Transcriptome profile analysis of two different regulatory T cell (T reg) populations (CD25 High and CD25 Low) from mice which lack autophagy in their DC (Atg5 -/- DC) and their litter mate controls (Atg5 +/+ DC). Samples were run in triplicates. cDNA were used for library preparation using the Nextera XT kit from Illumina