Table S1 - Prevalence and Infection Load Dynamics of Rickettsia felis in Actively Feeding Cat Fleas

Raw data for gene copy numbers of (a) R. felis 17-kDa and (b) C. felis 18S rDNA in individual flea samples. In three independent trials spanning one year, individual fleas were assessed by qPCR for R. felis infection prior to the start of the feeding (Day 0) and during nine days of feeding on cat hosts. (a) R. felis 17-kDa gene copy number quantities per individual flea lysate were extrapolated based on their position relative to the standard curve and listed in the above table. Individual fleas negative for infection with R. felis are represented by ��-��. (b) C. felis 18S rDNA gene copy number quantities per individual flea lysate were also determined by extrapolating their values based on their position relative to the standard curve and are listed in the above table. C. felis 18S rDNA counts were performed for all fleas, however, fleas determined to be infected with R. felis are shaded in gray. The values in the above tables were log transformed and ratios of logRf17kDa/logCf18SrDNA were generated for individual fleas samples (results presented in main text). (0.04 MB PDF)