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Multiplex indexing approach for the detection of DNase I hypersensitive sites in single cells

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NIAID Data Ecosystem2026-04-30 收录
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We describe a novel indexing protocol that employs the activities of terminal DNA transferase and T4 DNA ligase to add unique cell barcodes to DNase-digested DNA ends. This protocol allows profiling of genome-wide DHSs in over 10,000 single-cells in an individual experiment. Overall design: Profiling genome-wide DHSs with terminal DNA transferase and T4 DNA ligase

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2022-11-11
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