Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells - 2019/01/28
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<strong>Project: </strong>Biophysical investigation of purified HTT protein samples <strong>Experiment: </strong>Expression and purification of HTT<sup>79-3144 </sup>with and without HAP40 from Sf9 Insect cells <strong>Date completed: </strong>2019/01/28 <strong>Rationale: </strong>HTT lacking the unstructured region of exon1 (aa. 1-78 which are not resolved in the cryoEM model PDB ID 6EZ8) may serve as a useful control when determining exon1-dependent functionality of the HTT protein. Apo and HAP40-bound HTT<sup>79-3144 </sup>will be produced from Sf9 insect cell expression system.
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2019-03-19



