Gene expression profiling of shCtrl and shGABRD AGS cells from Clariom S array

GABRD is identified as a tumor promotor in the development of gastric cancer. Herein, to further explore the downstream regulated genes involved in GABRD mediated tumor progression, an Affymetrix Clariom S human Assay was performed to profile the expression of GABRD regulated genes in AGS cells. Gene expression profiling of shCtrl and shGABRD AGS cells was acquired and analyzed. Differentially expressed genes were identified based on fold change of mean of expression (fold change ≥ 1.5) and FDR (< 0.05) from P value calculated based on linear model of empirical Bayesian distribution. GABRD expression regulated the expression of 2644 genes in AGS cells, of which 1199 were upregulated and 1445 were downregulated upon GABRD silencing. Gene expression in shGABRD (GABRD knockdown) AGS cells and shCtrl (negative control) AGS cells was detected using Human gene expression array. Gene chip was performed with Clariom S array according to the instructions.