Table2_Selection of Tropical Microalgae Species for Mass Production Based on Lipid and Fatty Acid Profiles.pdf

Numerous recent studies have identified microalgae biofuel as one of the major renewable energy sources for sustainable development due to their high biomass productivity, high lipid content, and availability of locally adapted strains in various geographical locations. There have been minimal studies on the fatty acid composition of lipid production on local microalgae species in Sabah, Malaysia. Thus, screening for local microalgae species capable of producing biodiesel can aid in the selection of suitable species. This study aimed to isolate and identify promising local microalga as biodiesel feedstock for mass cultivation. Eight microalgae species, Acutodesmus obliquus, Chaetoceros muelleri, Isochrysis galbana, Ankistrodesmus falcatus, Chlamydomonas monadina, Chlorella emersonii, Nannochloropsis oculata, and Tetraselmis chuii, were successfully isolated and identified from Kota Kinabalu, Sabah. The isolated microalgae were characterized based on the lipid/biomass productivity, lipid content and fatty acid profiles. These isolates had biomass productivity of 0.11–0.78 g/L/day, lipid content of 11.69–39.00% dry weight, and lipid productivity of 21.11–252.64 mg/L/day. According to GC-MS analyses, four isolates produced more than 80% of C14–C18 fatty acids, which were A. falcatus (95%), C. emersonii (93%), A. obliquus (91%), and C. muelleri (81%). Despite its low biomass productivity, C. muelleri was chosen as the best biodiesel species candidate because of its moderately high lipid productivity (42.90 mg/L/day), highest lipid content (39% dry weight), high level of MUFAs and C14–C18 FAs (81.47%), with the highest oleic acid proportion (28.38%), all of which are desirable characteristics for producing high-quality biodiesel.

诸多近期研究已将微藻生物燃料确认为可持续发展的主要可再生能源之一，这得益于其高生物量生产力、高脂质含量以及在不同地理区域中本地适应菌株的可用性。关于马来西亚沙巴地区本地微藻物种脂质生产中脂肪酸组成的探讨则相对较少。因此，筛选能够生产生物柴油的本地微藻物种有助于适宜物种的选择。本研究旨在从沙巴州哥打基纳巴卢成功分离和鉴定出具有生产生物柴油潜力的本地微藻，以供大规模养殖。八种微藻物种，包括Acutodesmus obliquus、Chaetoceros muelleri、Isochrysis galbana、Ankistrodesmus falcatus、Chlamydomonas monadina、Chlorella emersonii、Nannochloropsis oculata以及Tetraselmis chuii，均被成功分离和鉴定。所分离的微藻根据脂质/生物量生产力、脂质含量及脂肪酸谱进行了特征化分析。这些分离菌株的生物量生产力为0.11–0.78 g/L/天，脂质含量为11.69–39.00%干重，脂质生产力为21.11–252.64 mg/L/天。根据气相色谱-质谱联用（GC-MS）分析，四种菌株产生了超过80%的C14–C18脂肪酸，分别为A. falcatus（95%）、C. emersonii（93%）、A. obliquus（91%）和C. muelleri（81%）。尽管其生物量生产力较低，但C. muelleri因其适中的脂质生产力（42.90 mg/L/天）、最高的脂质含量（39%干重）、高水平的单不饱和脂肪酸（MUFA）和C14–C18脂肪酸（81.47%），以及最高的油酸比例（28.38%），均具有生产高品质生物柴油的理想特性，因此被选为最佳生物柴油物种候选者。