Effect of DEN-induced liver cancer in wildtype and OTUD3 knockout mice

To further investigate gene expression in DEN-induced liver cancer in mice with Otud3 gene knockout, we employed RNA-seq profiling as a discovery platform to identify the pathways or biological processes potentially responsible for the occurrence of DEN-induced liver cancer in both wildtype and Otud3 knockout mice. Liver cancer tissues from Otud3 knockout mice and their littermates, with or without a 14-day-old DEN injection, were utilized as samples for analysis. Overall design: RNA-seq in mice liver cancer tissues was measured at 30 weeks post-DEN-injection. Three independent mice for each group were performed.