Data from: Study on expression modes and cleavage role of miR156b/c/d and its target gene Vv-SPL9 during the whole growth stage of grapevine

miR156 regulates the expression of its target SPL (PROMOTER BINDING-LIKE) genes during flower and fruit development, diverse developmental stage transitions, especially from vegetative to reproductive growth phases, by cleaving the target mRNA SPL of one plant-specific transcription factor. However, systematic reports on grapevine have yet to be presented. Here, the precise sequences of miR156 (vvi-miR156b/c/d) in grapevine ‘Takatsuma’ was cloned with a previously cloned grapevine SPL (Vv-SPL9). Their expression profiles in 18 grapevine tissues were identified through stem-loop RT-PCR. The interaction mode between vvi-miR156b/c/d and Vv-SPL9 was further validated by detecting the cleavage site and cleavage products of 3′- and 5′-ends via an integrated approach of 5′-RLM-RACE (RNA ligase-mediated 5′-rapid amplification of cDNA ends), 3′-PPM-RACE (poly(A) polymerase-mediated 3′-rapid amplification of cDNA ends), and qRT-PCR (real time reverse transcriptase-polymerase chain reaction). The variation in their cleavage roles in the whole growth stage of grapevine was also systematically investigated. Results showed that vvi-miR156b/c/d exhibited typical temporal-spatial specific expression levels. The expression levels were higher in vegetative organs, such as leaf, than in reproductive organs, such as tendrils, flowers, and berries. A significant variation was observed during vegetative-to-reproductive transition. The expression patterns of Vv-SPL9 showed the opposite trends with those of vvi-miR156b. We confirmed that their cleavage site was at the 10th site of vvi-miR156b/c/d complementary to Vv-SPL9 in ‘Takatsuma’ grapevine. We also identified the temporal-spatial variation of their cleavage products. This variation can indicate the regulatory function of miR156 on SPL in grapevines.