five

Creation of streptomycin-dependent mycobacteria via recombineering

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https://www.ncbi.nlm.nih.gov/sra/SRP547971
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Mycobacterium smegmatis mc(2)155 and Mycobacterium tuberculosis H37Rv were made streptomycin-dependent using oligonucleotide mediated recombineering. Several spontaneously streptomycin-dependent strains of M. tuberculosis H37Rv were also discovered during this project. The streptomycin-dependent phenotype was used for selection and counterselection to recombineer other mutations into M. smegmatis.
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2025-12-01
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