H3K36me3 protects the mouse epigenome from single nucleotide variations [ChIP-Seq]

We analyze high-throughput profiling of histone H3K36me3 in mouse embryonic stem cells from chromatin immunoprecipitated DNA captured by H3K36me3-specific antibody. We find that H3K36me3 distribution is like expected and enriched at transcribing gene bodies especially with enrichment at 3'-terminal of gene body, which is identical with findings observed in other mammalian cells. Overall design: Examination of histone H3K36me3 modification in mouse embryonic stem cell with histone H3.1WT overexpression, we analyzed two replicates.