sequencing of activated sludge samples from a French start-up WWTP to monitor the evolution of ecosystems over time. Pegasus sequencing

The Seine-Morée wastewater treatment plant (SM_WWTP), with a capacity of 100 000 population-equivalents, was fed with raw domestic wastewater all over its start-up phase. Its microbiome resulted from the spontaneous evolution of wastewater borne microorganisms. This rare opportunity allowed us to analyze the sequential microbiota colonization and implantation follow up during the start-up phase of this WWTP by the mean of a regular sampling carried out over 8 months until the establishment of a stable and functional ecosystem.During the study, biological nitrification-denitrification and dephosphatation appeared 68 days after the start-up of the WWTP, while flocs decantation occurred 91 days later. High throughput sequencing of 18S and 16S rRNA genes was performed using Illumina's MiSeq and PGM Ion Torrent platforms respectively, generating 584,647 16S and 521,031 18S high-quality sequence rDNA reads.