Diagnosis of childhood bacterial and viral infection using host RNA expression - Meningococcal validation cohort

Genome-wide analysis of transcriptional profiles in children <17 years of age with bacterial or viral infections or with clinical features suggestive of infection. Patients were recruited at or after presentation to hospital. Blood was collected into PAX gene tubes (PreAnalytiX). Total RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labeled cRNA was hybridized to Illumina Human Ref8 Beadchips. Numbers of arrays analysed were as follows: Meningococal disease (n=24), Healthy adult controls (n=21)