CD300E is activated by Helicobacter pylori macrophages infection

Macrophages are important cells involved in infections responses. They are mediators of gastritis in acute Helicobacter pylori (Hp) infection. After macrophages infection with Hp one of the most up-regulated protein is CD300E. To identify effects of CD300E activation in macrophages an agonistic antibody was used to activate CD300E and effects on gene expression were monitored. A time serie experiment was performed to identify effects of CD300E activation. 23 samples were profiled. Macrophages monocytes derived using M-CSF for 6 days. They were cultured for 1 d in culture medium without M-CSF and then infected with Helicobacter pylori for 48h. After infection macrophages were treated with an antibody to activate CD300E for 3 hours (3 biological replicates), 6 hours (4 biological replicates) and 24 hours (4 biological replicates). As controls were used macrophages not treated with the activacting CD300E antibody: 3, 6, and 24 hours four biological replicates each.